Table 1.
Primer sequences used for PCR amplification of chicken interleukin-6 (IL-6).
| Primer | Starting Position | Primer Sequence (5′ → 3′) | Annealing Temperature (°C) | Length (bp) |
|---|---|---|---|---|
| P1 | −2210 | F: AGAGAGGACTAACCCACAGAG | 58.5 | 698 |
| R: CCAGCTTCTCCAGTCTTGTC | ||||
| P2 | −1628 | F: AGGGACAGCAATGGCAGAAG | 60.5 | 717 |
| R: AAGAGCTGATCCTGGTTCTGG | ||||
| P3 | −994 | F: CAGAGGACGTCCTACCTCAA | 56.5 | 690 |
| R: GGTGAGCCTGGCAGCC | ||||
| P4 | −367 | F: AAGATAAGACGCGCCACACC | 58.5 | 803 |
| R: TTGAGGTTGTTCCGGACGAG | ||||
| P5 | 391 | F: CGTGTGCGAGAACAGCAT | 59.0 | 565 |
| R: AAATAGAAAGTTGGAAGGAGAGTACA | ||||
| P6 | 797 | F: GGTGTGCAGTCGGCAATAG | 57.5 | 803 |
| R: CTCATCATTCCACACAAGG | ||||
| P7 | 1388 | F: ACTGTGTGCTCCTAATGCCT | 60.5 | 770 |
| R: CTTCAGATTGGCGAGGAGGG | ||||
| P8 | 1989 | F: CGTACCTCAAAACCTACCTCAG | 59.0 | 768 |
| R: AGTGGAGTTCTTCAGCCTTATTTAA | ||||
| P9 | 2702 | F: CAAAACCAACCTGTCTAAGCTG | 59.5 | 773 |
| R: TTTTAAGAACTGTATGTTGATTGTGC |
Bp: base pairs, F: Forward, R: Reverse.