Table 1.

IC₅₀ value of HNTX-I analogues determined by whole-cell patch clamp *.

Peptide	Amino Acid Sequence	hNaV1.7 IC50 (µM)
Native HNTX-I	ECKGFGKSCVPGKNECCSGYACNSRDKWCKVLL	>10
E1G	GCKGFGKSCVPGKNECCSGYACNSRDKWCKVLL	>10
N23S	ECKGFGKSCVPGKNECCSGYACSSRDKWCKVLL	0.435 ± 0.072
D26H	ECKGFGKSCVPGKNECCSGYACNSRHKWCKVLL	1.498 ± 0.093
L32W	ECKGFGKSCVPGKNECCSGYACNSRDKWCKVWL	>10
N23S–D26H	ECKGFGKSCVPGKNECCSGYACSSRHKWCKVLL	0.079 ± 0.004
E1G–N23S–D26H	GCKGFGKSCVPGKNECCSGYACSSRHKWCKVLL	0.179 ± 0.024
E1G–R25K–D26H	GCKGFGKSCVPGKNECCSGYACNSKHKWCKVLL	0.766 ± 0.029
N23S–D26H–L32W	ECKGFGKSCVPGKNECCSGYACSSRHKWCKVWL	0.071 ± 0.005
E1G–R25K–D26H–L32W	GCKGFGKSCVPGKNECCSGYACNSKHKWCKVWL	0.669 ± 0.070
E1G–N23S–D26H–L32W	GCKGFGKSCVPGKNECCSGYACSSRHKWCKVWL	0.036 ± 0.007

*—Data are presented as mean ± SEM, n ≥ 3. Amino acid residues in red color represented the mutated residues.