Figure 5.

siRNA silencing of lamin A/C in HDLM-2. Lamin A/C expression was monitored by Western Blot after siRNA lamin A/C transfection in different concentration (12.5 nM, 25 nM and 50 nM) for 24 h (A), 48 h (B), 72 h (C) and 96 h (D). siRNA Scramble was used as a negative control and Cyclophilin B was used as a loading control. The fold-decrease for the siRNA is relative to the negative control (Scramble for each concentration) and the Scramble is relative to control without transfection (Control). These experiments were performed in triplicate using cells from different passages.