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. 2018 Sep 1;10(9):351. doi: 10.3390/toxins10090351

Figure 2.

Figure 2

Isc stimulated by EspP in proximal HCM is concentration dependent and EspP-specific. (A) EspP-stimulated Isc increases with each sequential 6 µg/mL apical addition of EspP (blue arrows). (B) Representative Isc traces show that apically added purified recombinant EspP (6 µg/mL; red traces) as well as lysate from AD202 bacteria containing EspP plasmid (30 µL, blue traces), but not lysate from AD202 parental strain lacking either EspP or EspP S263A plasmid (30 µL, black traces), rapidly increase Isc. (C) Representative Isc traces show that pre-absorbing EspP toxin with specific anti-EspP antibodies reduced the EspP-increase in Isc by ~70% (red traces); black trace—time control; blue trace—purified EspP. Blue arrows indicate the time point when toxins or lysates were added apically.