Figure 9.

Phenotypes of PaPro22^Δ and Papro45^Δ. (A) The wild type (WT), PaPro22^Δ and Papro45^Δ were inoculated at the same time on M2 plates and the pictures were taken ten days later. PaPro22^Δ and Papro45^Δ have a slow growth, are devoid of aerial hyphae, but accumulate pigments. (B) CG test were made as those of Figure 1 and Figure 7. Apical hyphae of PaPro22^Δ and Papro45^Δ show after 3–4 cm the arrest of growth and accumulation of dark pigments typical of senescence. (C) Sexually compatible mycelia of the indicated genotypes were inoculated on M2 and three days later 2 mL of water were added to spread spermatia. The wild-type thalli differentiated after seven days mature perithecia, but not the mutant ones, showing that PaPro22^Δ and Papro45^Δ were ♀-sterile and ♂-fertile. Perithecia are the small black dots. (D) Anastomoses (open circle) are frequent in the wild-type, but very rare in PaPro22^Δ and Papro45^Δ (a field in which one figure of anastomose was seen is shown for the Papro45^Δ mutant, in most fields anastomoses could not be observed). Hyphae from PaPro22^Δ and Papro45^Δ often meet, but do not engage cell fusion (arrows).