Table 4.
Serum biochemistry parameters associated immune function for dairy cows offered fermented Chinese herbal medicine (CHMs)
| IL-1 | IL-2 | IL-6 | IL-12 | IgA | IgG | IgM | Bak | Mcl-1 | Bax | Bcl-2 | Bcl-xl | P53 | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Variable | (pg/mL) | (pg/mL) | (pg/mL) | (pg/mL) | (µg/mL) | (µg/mL) | (µg/mL) | (ng/mL) | (pg/mL) | (ng/mL) | (ng/mL) | (ng/mL) | (pg/mL) | |
| D 0 CHMs dose, g/d |
0 | 66.6 | 853.4 | 151.9 | 546.7 | 318.1 | 278.7 | 126.6 | 10.27 | 1181.3 | 4.46 | 81.7 | 10.04 | 536.3 |
| 25 | 69.3 | 846.3 | 162.0 | 556.4 | 309.5 | 285.1 | 133.6 | 10.91 | 1199.5 | 4.44 | 75.5 | 9.83 | 546.2 | |
| 50 | 74.6 | 840.1 | 145.5 | 540.9 | 311.9 | 280.6 | 132.0 | 10.70 | 1152.1 | 4.53 | 85.5 | 10.90 | 568.1 | |
| 100 | 63.8 | 839.31 | 156.5 | 557.4 | 315.5 | 282.6 | 130.0 | 10.68 | 1188.2 | 4.38 | 76.4 | 10.75 | 529.6 | |
| SEM | 2.76 | 6.61 | 4.56 | 13.56 | 4.42 | 7.94 | 4.20 | 0.42 | 65.77 | 0.19 | 2.86 | 0.47 | 31.57 | |
| D 14 CHMs dose, g/d |
0 | 67.6 | 906.3 | 152.6 | 526.7 | 311.0 | 283.6 | 126.3 | 12.22 | 1344.1 | 4.81 | 73.4 | 9.95 | 534.1 |
| 25 | 53.6 | 882.9 | 137.5 | 521.6 | 311.5 | 285.0 | 131.6 | 13.29 | 1190.5 | 4.83 | 80.2 | 10.29 | 535.2 | |
| 50 | 52.1 | 914.7 | 140.0 | 536.3 | 323.2 | 293.5 | 124.4 | 13.32 | 1346.3 | 4.87 | 80.1 | 9.13 | 613.2 | |
| 100 | 52.0 | 953.0 | 160.7 | 529.5 | 317.0 | 292.9 | 124.1 | 11.58 | 1062.9 | 3.54 | 86.1 | 11.58 | 563.7 | |
| SEM | 4.07 | 8.15 | 5.19 | 17.79 | 5.06 | 7.54 | 7.02 | 0.45 | 68.03 | 0.21 | 2.43 | 0.36 | 28.79 | |
| D 28 CHMs dose, g/d |
0 | 58.7 | 950.5 | 150.1 | 535.9 | 302.2 | 278.0 | 127.7 | 10.49 | 1387.0 | 4.95 | 73.2 | 10.57 | 523.2 |
| 25 | 49.5 | 985.7 | 138.6 | 541.3 | 301.7 | 295.4 | 135.0 | 11.85 | 1423.1 | 3.97 | 79.5 | 10.29 | 467.0 | |
| 50 | 48.9 | 1066.0 | 139.4 | 537.0 | 325.3 | 318.6 | 128.4 | 10.87 | 1398.3 | 3.00 | 78.4 | 11.36 | 515.3 | |
| 100 | 53.9 | 1014.9 | 151.0 | 542.9 | 310.1 | 317.7 | 118.1 | 10.23 | 1123.3 | 2.90 | 94.8 | 10.99 | 611.0 | |
| SEM | 2.92 | 18.50 | 4.15 | 11.33 | 7.26 | 7.75 | 2.45 | 0.50 | 59.94 | 0.22 | 2.11 | 0.39 | 36.72 | |
| D 42 CHMs dose, g/d |
0 | 65.7 | 1016.4 | 146.2 | 496.0 | 305.4 | 259.9 | 119.0 | 10.56 | 1272.3 | 4.52 | 77.6 | 9.17 | 558.0 |
| 25 | 47.6 | 1035.5 | 142.3 | 521.0 | 303.6 | 269.7 | 124.3 | 10.24 | 1301.8 | 3.70 | 85.7 | 10.98 | 357.9 | |
| 50 | 47.6 | 1145.3 | 150.1 | 550.0 | 311.6 | 323.8 | 132.6 | 9.79 | 1612.6 | 3.32 | 93.1 | 12.24 | 458.2 | |
| 100 | 41.1 | 1149.8 | 197.5 | 562.7 | 311.7 | 338.1 | 138.1 | 8.96 | 1467.1 | 2.76 | 106.3 | 12.22 | 320.3 | |
| SEM | 3.42 | 14.96 | 5.09 | 12.24 | 9.74 | 8.96 | 5.72 | 0.24 | 38.15 | 0.25 | 2.28 | 0.46 | 23.78 | |
| P-value | 1D | 0.08 | 0.02 | 0.01 | 0.48 | 0.71 | 0.01 | 0.84 | 0.17 | 0.31 | 0.03 | 0.05 | 0.25 | 0.29 |
| 2P | 0.04 | <0.01 | 0.21 | 0.75 | 0.89 | 0.36 | 0.95 | <0.01 | 0.05 | <0.01 | <0.01 | 0.38 | 0.03 | |
| 3D×P | 0.60 | 0.08 | 0.21 | 0.98 | 0.98 | 0.46 | 0.94 | 0.96 | 0.43 | 0.10 | 0.06 | 0.45 | 0.44 |
1D = dose effect: 0, 25, 50, or 100 g CHMs supplement per cow per day.
2P = period effect: experimental period for 14, 28, and 42 d.
3D × P = interaction between dose and period.