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. 2018 Jul 19;96(10):4159–4172. doi: 10.1093/jas/sky278

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Figure 4. — Effect of tea polyphenol (TP) on hydrogen peroxide (H₂O₂)-induced nuclear factor-erythroid 2-related factor 2 (NFE2L2) protein and mRNA abundance. (A, B) NFE2L2 protein and mRNA abundance in control bovine mammary epithelial cells (BMEC) or BMEC receiving H₂O₂ and TP with or without H₂O₂. The BMEC were pretreated with or without 100 µg/mL of TP for 12 h and then treated with or without 600 µM of H₂O₂ for 6 h. The NFE2L2 protein and mRNA abundance were analyzed by Western blot and quantitative real-time PCR. Means with different superscript letters differ (P < 0.05). There were 5 replications for each experiment and each well.