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. 2018 Jul 19;96(10):4159–4172. doi: 10.1093/jas/sky278

Figure 7.

Figure 7.

Effect of tea polyphenol (TP) treatment on protein abundance of nuclear factor-erythroid 2-related factor 2 (NFE2L2) signaling pathway proteins in bovine mammary epithelial cells (BMEC) under control and oxidative stress (OS) conditions. (A to E) Protein abundance of NFE2L2, heme oxygenase-1 (HMOX-1), NAD(P)H:quinone oxidoreductase 1 (NQO1), glutamate-cysteine ligase catalytic subunit (GCLC), and glutamate-cysteine ligase modifier subunit (GCLM) in BMEC under control and OS. The BMEC were preincubated with a MAPK-specific inhibitor, 2,4-dinitrochloro-benzene (DNCB), for 30 min and then treated with or without TP (100 µg/mL) for another 12 h, followed by hydrogen peroxide (H2O2; 600 µM) exposure for 6 h. The protein abundance of NFE2L2, HMOX-1, NQO1, GCLC, and GCLM was measured by Western blot. Means with different superscript letters differ (P < 0.05). There were 5 replications for each experiment and each well.