Table 3.

Effect of different concentrations and incubation times with tea polyphenols (TP) on the proliferation rate of bovine mammary epithelial cells (BMEC)¹

	Time (h)
TP concentration (µg/mL)	0	6	8	12	24	SEM	P-value
0	100.00a2A3	100.00aA	100.000bA	100.000bA	100.000bA	0	—
60	101.278aD	103.068aCD	110.168aBC	119.384aA	115.714aAB	2.834	0.0007
80	103.192aB	106.068aB	115.048aA	119.432aA	117.090aA	2.458	0.0004
100	105.514aB	107.740aB	117.264aA	120.060aA	119.476aA	3.046	0.0054
120	90.438bA	92.430aA	94.106bA	97.064bA	95.224bA	6.102	0.9491
SEM	2.616	5.582	2.919	2.33	2.942
P-value	0.0065	0.3527	<0.0001	<0.0001	<0.0001

¹BMEC proliferation rate in response to increasing concentrations of TP (0 to 120 µg/mL) without hydrogen peroxide (H₂O₂). The BMEC were pretreated with 0 to 120 µg/mL of TP for 0 to 24 h prior to analysis of proliferation rate. Proliferation rate was measured with 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS). There were 5 replications for each experiment and each well.

²Similar lowercase letters for means in the same column denote lack of significant differences (P > 0.05). Different lowercase letters for means in the same column differ (P < 0.05).

³Similar capital letters for means in the same row denote lack of significant differences (P > 0.05). Different lowercase letters for means in the same row differ (P < 0.05).