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. 2018 Aug 30;7(9):116. doi: 10.3390/antiox7090116

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Gel band shift analysis of PBS-soluble human umbilical vein endothelial cell (HUVEC) proteins. Oxidized palmitoyl-arachidonoyl-phosphatidylcholine (OxPAPC) incubated with proteins for 90 min significantly changed the electrophoretic protein profile (lane 3). OxPAPC which is not covalently bound to proteins does not influence the electrophoretic mobility of proteins (lane 2). Lane 1: control sample time point 0; lane 4: control sample time point 90. The final OxPAPC concentration in treated samples was 100 µmol/L. Main visible changes are marked with asterisks.