Table 7.
Optimized ESI-MS/MS conditions for analytical method.
| Compound | Precursor Ion (m/z) | Declustering Potential (V) | Product ions (m/z) * | Collision Energy (V) | Cell Exit Potential (V) |
|---|---|---|---|---|---|
| Patulin | 152.9 | −55 | 108.9/81.0 | −12/−16 | −5/−3 |
| Nivalenol | 371.1 | −60 | 281.0/59.0 | −22/−38 | −5/−1 |
| Deoxynivalenol−3-Glucoside | 517.2 | −70 | 427.1/457.2 | −28/−20 | −9/−11 |
| Deoxynivalenol | 355.1 | −55 | 265.0/59.0 | −22/−38 | −11/−10 |
| Fusarenon X | 413.1 | −35 | 263.1/186.9 | −22/−36 | −10/−11 |
| Deepoxy-deoxynivalenol | 339.1 | −50 | 249.2/59.0 | −16/−34 | −15/−10 |
| α-Zearalanol | 321.1 | −110 | 277.0/303.1 | −30/−30 | −5/−7 |
| β-Zearalanol | 321.1 | −110 | 277.0/303.1 | −30/−30 | −5/−7 |
| β-Zearalenol | 319.0 | −110 | 159.8/173.9 | −42/−36 | −11/−11 |
| α-Zearalenol | 319.0 | −110 | 159.8/173.9 | −42/−36 | −11/−11 |
| Zearalanon | 319.0 | −105 | 204.8/161.0 | −32/−38 | −13/−9 |
| Zearalenon | 317.1 | −110 | 131.0/174.9 | −40/−34 | −7/−11 |
| T2 Tetraol | 361.2 | 56 | 215.0/281.1 | 13/13 | 14/20 |
| Neosolaniol | 400.2 | 51 | 185.1/245.1 | 29/17 | 12/16 |
| 15-Acetyldeoxynivalenol | 356.1 | 41 | 321.0/137.1 | 19/21 | 22/8 |
| 3-Acetyldeoxynivalenol | 339.2 | 71 | 231.1/203.1 | 17/21 | 16/14 |
| Monoacetoxyscirpenol | 342.1 | 41 | 265.1/107.1 | 13/21 | 18/6 |
| Diacetoxyscirpenol | 384.2 | 51 | 307.0/247.0 | 17/21 | 20/16 |
| Aflatoxin M1 | 329.0 | 71 | 273.1/259.0 | 33/33 | 18/18 |
| Aflatoxin B1 | 313.1 | 106 | 285.1/128.1 | 33/93 | 20/8 |
| Aflatoxin B2 | 315.1 | 106 | 287.1/259.0 | 37/41 | 20/18 |
| Aflatoxin G1 | 329.1 | 96 | 242.9/200.1 | 37/57 | 16/14 |
| Aflatoxin G2 | 331.0 | 76 | 189.0/245.1 | 59/45 | 13/12 |
| Fumonisin B1 | 722.4 | 116 | 334.4/352.3 | 57/51 | 10/10 |
| Fumonisin B2 | 706.4 | 121 | 336.4/318.3 | 51/55 | 10/10 |
| Fumonisin B3 | 706.4 | 121 | 336.4/318.3 | 51/55 | 10/10 |
| T2 Triol | 400.2 | 36 | 215.2/233.1 | 17/13 | 14/16 |
| Roquefortine C | 390.2 | 81 | 193.0/322.0 | 39/29 | 12/22 |
| Griseofulvin | 353.1 | 71 | 165.0/215.1 | 29/29 | 10/14 |
| T−2 toxin | 484.2 | 56 | 215.0/305.1 | 29/21 | 14/8 |
| HT−2 toxin | 442.2 | 61 | 215.1/263.0 | 19/19 | 14/18 |
| Ochratoxin A | 404.1 | 61 | 239.0/358.1 | 33/21 | 16/10 |
| Ochratoxin B | 370.1 | 61 | 205.0/187.1 | 29/49 | 14/12 |
| Mycophenolic acid | 321.1 | 66 | 207.0/303.0 | 29/15 | 14/20 |
| Mevinolin | 405.3 | 81 | 285.1/199.1 | 17/17 | 20/12 |
Source temperature: 500 °C; curtain gas 20 psi; ion source gas 1 (sheath gas) 50 psi; ion source gas 2 (drying gas) 50 psi, ion spray voltage −4000 V (negative polarity) and 4500 V (positive polarity). * values are given in the order quantifier/qualifier.