. 2018 Aug 29;96(10):4339–4347. doi: 10.1093/jas/sky292

Table 5.

Effect of cysteamine hydrochloride on the gene expression of cytoskeleton and tight junction in the intestinal mucosa of broiler chickens challenged with Clostridium perfringens

			PC + CSH, g/kg of diet				Dose trends (P-values)
Items	NC	PC	100	150	200	SEM	Linear	Quadratic
Cytoskeleton (mRNA expression, 2^−ΔΔCt)
TUBA1C	4.08^a	2.74^d	3.36^c	3.56^b	3.54^bc	0.038	<0.001	0.001
TUBB6	3.71^a	2.47^c	3.01^b	2.98^b	3.04^b	0.073	0.699	0.492
CAPZA1	2.80^a	1.13^c	2.18^b	2.20^b	2.28^b	0.039	0.095	0.596
CAPZA2	4.19^a	2.90^d	3.35^c	3.43^bc	3.62^b	0.047	0.002	0.340
SYNC	1.51^a	0.86^c	1.33^b	1.34^b	1.47^a	0.018	<0.001	0.040
SYNM	1.99^a	0.43^d	0.74^c	1.20^b	1.28^b	0.071	<0.001	0.022
Tight junction (mRNA expression, 2^−ΔΔCt)
CLDN1	3.76^a	0.70^d	2.08^c	2.52^b	2.66^b	0.050	<0.001	0.024
ZO-2	0.65^a	0.42^d	0.50^c	0.56^b	0.56^b	0.008	<0.001	0.013

^a–dMeans within a row with no common superscripts are significantly different (P < 0.05).

CAPZA, actin protein of muscle Z-line alpha; CLDN1, claudin-1; CSH, cysteamine hydrochloride; NC, negative control, without C. perfringens challenge; PC, positive control, challenged with C. perfringens; SYNC, syncoilin; SYNM, synemin; TUBA1C, tubulin alpha 1c; TUBB6, tubulin beta 6; ZO-2, zona occludens protein-2.