Table 1.
Phenotypic assays of C. neoformans and C. gattii clinical and environmental isolates inoculated in the invertebrate virulence model of G. mellonella. Association with sequence type (ST).
| Species Complex | ST | Strain (HOO58-I-) | Colony Morphology | Cellular and Capsular Size in µm | ||||
|---|---|---|---|---|---|---|---|---|
| Texture | Appearance | Pre-Inoculation | Post-Inocultion | |||||
| Cellular | Capsular | Cellular | Capsular | |||||
| C. neoformans | Clinical | |||||||
| 2 | 3746 | Mucoid | Smooth | 7.53 | 0.21 | 0.52 | 4.49 | |
| 2 | 3852 | 5.34 | 0.48 | 1.60 | 6.98 | |||
| 5 | 2881 | 5.57 | 0.52 | 1.76 | 4.58 | |||
| 6 | 3463 | 5.68 | 0.50 | 2.88 | 4.05 | |||
| 25 | 3104 | 5.49 | 0.75 | 0.49 | 6.14 | |||
| 32 | 2340 | Non-mucoid | 5.87 | 0.48 | 0.56 | 5.42 | ||
| 40 | 3589 | Mucoid | 6.20 | 0.48 | 1.63 | 4.40 | ||
| 63 | 2503 | 5.75 | 0.52 | 0.87 | 4.48 | |||
| 69 | 3099 | 5.87 | 0.45 | 1.81 | 5.96 | |||
| 71 | 3489 | 3.30 | 0.34 | 1.60 | 5.22 | |||
| 77 | 708 | 6.71 | 1.14 | 1.03 | 6.40 | |||
| 77 | 3845 | 5.72 | 0.67 | 1.60 | 4.35 | |||
| 93 | 2624 | Non-mucoid | Wrinkled | 6.53 | 0.45 | 1.61 | 11.88 | |
| 93 | 3189 | Mucoid | Smooth | 6.72 | 0.59 | 1.25 | 11.51 | |
| 93 | 995 | 5.54 | 0.59 | 3.08 | 9.18 | |||
| 93 | 1226 | 5.99 | 0.57 | 0.86 | 6.39 | |||
| 93 | 2073 | 4.73 | 1.22 | 1.02 | 7.98 | |||
| 93 | 2356 | Non-mucoid | 6.09 | 0.55 | 1.10 | 6.84 | ||
| 93 | 3938 | Mucoid | 6.15 | 0.48 | 0.90 | 4.40 | ||
| 199 | 714 | 5.90 | 0.93 | 3.42 | 9.78 | |||
| 307 | 707 | 5.85 | 0.92 | 2.55 | 7.97 | |||
| 307 | 727 | 5.44 | 0.83 | 1.32 | 8.00 | |||
| 307 | 2087 | 6.19 | 0.85 | 3.24 | 10.33 | |||
| 307 | 2274 | 6.12 | 0.89 | 2.25 | 9.16 | |||
| Environmental | ||||||||
| 15 | 4419 | Mucoid | Smooth | 5.69 | 0.65 | 1.12 | 6.04 | |
| 23 | 4706 | 4.29 | 0.25 | 1.34 | 6.80 | |||
| 56 | 4630 | 5.33 | 0.36 | 2.56 | 4.85 | |||
| 77 | 4013 | 5.74 | 0.67 | 2.05 | 4.37 | |||
| 93 | 3877 | 6.12 | 0.50 | 0.91 | 4.23 | |||
| 93 | 4711 | 6.39 | 0.59 | 0.91 | 4.35 | |||
| 226 | 5353 | 1.70 | 0.29 | 0.91 | 4.35 | |||
| C. gattii | Clinical | |||||||
| 25 | 212 | Non-mucoid | Smooth | 4.49 | 0.68 | 11.10 | 2.25 | |
| 47 | 255 | Mucoid | 5.40 | 2.88 | 9.40 | 1.85 | ||
| 85 | 792 | 3.57 | 0.66 | 8.90 | 2.21 | |||
| 106 | 1510 | 6.93 | 2.76 | 7.60 | 0.90 | |||
| 25 | 2877 | 5.74 | 0.46 | 5.53 | 1.20 | |||
| 51 | 3286 | 5.64 | 0.33 | 4.27 | 2.10 | |||
| 58 | 3031 | 4.98 | 0.47 | 6.07 | 1.10 | |||
| 323 | 3146 | 6.45 | 0.83 | 6.69 | 6.10 | |||
| 324 | 3407 | 4.40 | 1.39 | 4.29 | 1.40 | |||
| Environmental | ||||||||
| 25 | 3526 | Mucoid | Smooth | 3.08 | 0.25 | 5.69 | 1.20 | |
| 75 | 3593 | 2.45 | 0.65 | 5.15 | 1.90 | |||
| 79 | 4064 | 1.86 | 0.29 | 4.26 | 1.60 | |||
| 79 | 3080 | 1.89 | 0.25 | 4.30 | 1.60 | |||
| 79 | 3874 | 3.09 | 0.29 | 4.28 | 1.50 | |||
Low capsule size (from 0.6 to 2.2 μm), medium capsule size (from 2.3 to 3.3μm) and high capsule size (from 3.3 to 4.2 μm).