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Fluorescence emission spectra of 2′R-OTA (1 µM) in presence of increasing concentrations of RSA (0–5 µM) in PBS (pH 7.4), using 295 nm ((A) ex. slit: 5 nm, em. slit: 5 nm) and 394 nm ((B) ex. slit: 5 nm, em. slit: 10 nm) excitation wavelengths. Fluorescence emission intensity of 2′R-OTA (1 µM) at 447 nm in the presence of increasing albumin concentrations (0–5 µM) using 295 nm (C) and 394 nm (D) excitation wavelengths.
