Figure 5.

Nlrp12 inhibits phosphorylation of Akt and p65 in CD4⁺ T cells. The cells were activated either with PMA/ionomycin for 10 min or with cross-linking anti-CD3 antibody (anti-CD3/IgG) for 20 min at 37 °C. (A) Samples were analyzed by SDS-PAGE and immunoblotting for phospho-Akt (S473) and phospho-p65. Total Akt and p65 expression in the same lysates were used to normalize the expression of phosphorylated molecule between samples; (B) The ratio of phosphorylated to total molecules were compared between WT and Nlrp12^−/− cells, n = 3, * p < 0.05.