Table 1.
Methods for Schizophrenia-induced pluripotent stem cell (SCZ-iPSC) generation and quality control.
| Ref. | Source | Factors | Met | N° | Auth | Karyo | Pluripotency |
|---|---|---|---|---|---|---|---|
| [75] | fibroblast | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
| [76] | [75] | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
| [77] | [75] | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
| [78] | keratinocyte | OKSM | LV | 1 | nd | G-B | ICC, PCR, PluriTest, EB |
| [79] | [75] | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
| [80] | [75] | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
| [81] | [75] | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
| [82] | [75] | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
| [83] | fibroblast | OKSM | RV | 2 | nd | G-B | ICC, PCR, EB |
| [70] | [75,77] | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
| [84] | [78] | OKSM | LV | 1 | nd | G-B | ICC, PCR, PluriTest, EB |
| [85] | fibroblast | APM | LV | na | na | na | ICC |
| [55] | fibroblast | OKSM | LV | dns | nd | G-B | ICC, PluriTest, EB |
| [86] | [75] | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
| fibroblast | OKSM | Sen | 2–3 | nd | G-B | PCR, FACS | |
| [87] | [75] | OKSML | LV | ≥5 | nd | G-B | ICC, Tera |
Abbreviations are: APM, reprogramming factors ASCL1, POU3F2, MYT1L; Auth, authentication; EB, embryoid body formation combined with ICC; dns, data not shown; Epi, episomal plasmid; G-B, chromosomal G-banding; ICC, immunocytochemistry; Karyo, karyotype; LV, lentiviral transduction; Met, methods; N°, numbers of independent clones per donor; na, non-applicable; nd, not determined; OKSM, reprogramming factors OCT4, KLF4, SOX2, MYC, OKSML plus Lin28; PCR, quantitative reversed transcribed polymerase chain reaction; PluriTest, a bioinformatic tool to asses pluripotency; Sen, Sendai virus; Ref, reference; RV, retroviral; Tera, teratoma formation.