Figure 5.

Gp120 increases the activity of glycolytic enzymes in glioma cells. Colorimetric/fluorometric pyruvate kinase (A), hexokinase (B) and glyceraldehyde 3-phosphate dehydrogenase (C) activity assays were performed in untreated U87, A172 and 965 glioma cells and the same cells continuously treated with gp120 for 7–10 days. Kinase activity was calculated as the amount of product (pyruvate, NADPH and NADH respectively) produced in a sample in 10 min. Mean ± S.E. and significant differences between control and gp120 treatment (*) are shown (p < 0.05). Unpaired t-tests were used to determine the significance between groups. Five independent experiments (n = 5) were used for statistical analysis.