Table 2.

Allele-specific polymerase chain reaction (AS-PCR) primers for COMT (Val158Met) genotyping.

Primer Direction	Nucleotide Change	Amino Acid Change	Primer Sequence	Annealing Tempt	Product Size
Forward F			5′-ACTGTGGCTACTCAGCTGTG-3′	56 °C	169 bp
Reverse R1	G allele	(Val158)	5′-GCATGCACACCTTGTCCTT-3′
Reverse R2	A allele	(Met158)	5′-GCATGCACCACCTTGTCCTTCAT-3′