Table 1.

Oligonucleotide primers used for real-time qPCR.

Gene	Forward	Reverse
GAPDH	5′-TGATGACATCAAGAAGGTGG	5′-TTACTCCTTGGAGGCCATGT
SNAP25	5′-ACCAGTTGGCTGATGAGTCG	5′-GTTCGTCCACTACACGAGCA
VAMP-2	5′-CCATAGAGGGAGGGTGTTGC	5′-GTCCCCACCCTTACCTTGAG
SV2A	5′-GAAGGTGGTGCATCCAGTGA	5′-AGGCCTAGCATGCCTTTGTT
SytI	5′-TCCTGACCTGCTGCTTTTGT	5′-GGGTTTTGCCACCCAATTCC
SytII	5′-CATTGGACCCGTGGACAACT	5′-AGAACGCCCACAGTAAGCTG

Accession numbers for the genes were: GAPDH (AB062273), SNAP25 (NM_003081.4), VAMP-2 (AF135372.1), SV2A (NM_014849.4), SytI (NM_005639.2) and SytII (NM_177402.4).