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Expression pattern of Hla in Newman and NCTC8325 S. aureus strains and role of Hla in hemolytic activity. (A) Western blot analysis of supernatant from overnight culture from NCTC 8325 and Newman wild type (WT) and hla null mutants; (B) Neutralization of purified Hla by the monoclonal antibody Can6 in rabbit RBC assay; (C,D) Hemolytic activity of supernatants of WT and hla null mutant of NCTC 8325 (C) and Newman (D) strains in rabbit RBC assay. EC50: 50% toxicity titer.
