Table 3.
Comparison of metagenomic and metatranscriptomic data (datasets XII and XIII)
Dataset XII | Dataset XIII | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
MTG% | MTX% | Rho | p | Ratio | MTG% | MTX% | Rho | p | Ratio | |
CutCD | 95.68* | 83.33 | 0.09 | 0.42 | 0.72 | 99.67* | 68.09 | 0.11 | 0.06 | 0.95 |
CntAB | 33.45* | 0.00 | nd | nd | nd | 14.79* | 0.00 | nd | nd | nd |
GrdH | 80.58* | 48.72 | 0.27 | 0.02 | 0.80 | 94.06* | 31.35 | 0.09 | 0.14 | 0.75 |
BaiA-I | 65.11* | 28.21 | 0.22 | 0.05 | 3.15 | 97.69* | 62.38 | 0.22 | < 0.01 | 1.20 |
DsrAB | 55.40 | 70.52* | 0.50 | < 0.01 | 3.92 | 87.78 | 92.76* | 0.31 | < 0.01 | 20.7 |
The percentage of samples harboring individual pathofunctions in metagenomes (MTG%, n = 278 (XII); n = 311 (XIII)) and metatranscriptomes (MTX%, n = 78 (XII); n = 305 (XIII)) as well as correlations between abundance and expression levels (Spearman’s rho and p value) based on matched MTG/MTX samples (n = 78 (XII); n = 304 (XIII)) are shown. Ratio refers to the median RNA/DNA results of matched samples where only pairs that showed values > 0 at both levels were considered. nd: not determined. Seven metatranscriptomic samples and one metagenome from dataset XIII were omitted due to low sequencing depth (< 105 reads). Significant differences (*p < 0.05) between abundance and expression based on generalized linear models are indicated