Table 2.
Primers for Sanger sequencing. No.1–10: Exon primers; No.11–20: Quantitative real-time primers; No.21–26: Breakpoint primers
| No. | Primer | Position | Sequence (5′-3′) |
|---|---|---|---|
| 1 | G6PC-1F | Exon1 | CACCACCAAGCCTGGAATAAC |
| 2 | G6PC-1R | CAGACATTGCGAGAGCGAATG | |
| 3 | G6PC-2F | Exon2 | GCATTCATTCAGTAACCC |
| 4 | G6PC-2R | AGACAGAAGCTGAGTGGA | |
| 5 | G6PC-3F | Exon3 | CACCTTTACTCCATTCTCTTTC |
| 6 | G6PC-3R | GTGCCACAACTCTTAATCAGCG | |
| 7 | G6PC-4F | Exon4 | CACTGAGAGCACCTAAGTTTGC |
| 8 | G6PC-4R | CTGATTACACACAGGATGTGG | |
| 9 | G6PC-5F | Exon5 | CATGTCACCCACTCCTCCAAAC |
| 10 | G6PC-5R | GTCACTTGCTCCAAATACCAGTG | |
| 11 | G6PC-1ForF | 5′-Flanking introns | TTTCACAGTCCTCCGTGACC |
| 12 | G6PC-1ForR | AGGGCTTCTATATCTTGAGCTTTC | |
| 13 | G6PC-1QF | Exon1 | TCCAGTCAACACATTACCTCCA |
| 14 | G6PC-1QR | TAAAGACGAGGTTGAGCCAGTC | |
| 15 | G6PC-2inF | Intron2–3 | AAGTTGGGACAAGGGAATCAGA |
| 16 | G6PC-2inR | CATTCTTAATTCCTCTACCCTGAGA | |
| 17 | G6PC-4QF | Exon4 | GCTGAAGGATCTGCACCTGT |
| 18 | G6PC-4QR | AGGGAGTCAGATCAGCCCAT | |
| 19 | G6PC-5QF | Exon5 | CAGCTTCGCCATCGGATTTT |
| 20 | G6PC-5QR | ACAATAGAGCTGAGGCGGAA | |
| 21 | G6PC-D1F | GTGGGGAAAATGCCTGAGGA | |
| 22 | G6PC-D2F | TTTTCACCCTTGGGAGCCTG | |
| 23 | G6PC-D3F | GGTCACCCTGTCCCACTAGA | |
| 24 | G6PC-D4F | CTCACCTGTTTTCCCACGGA | |
| 25 | G6PC-D5F | GGGAGGAGACTCCAGGTCAT | |
| 26 | G6PC-comR | Intron2–3 | CTTTCCAGTCTGTGCCTCCAT |