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. 2018 Sep 25;11:6217–6226. doi: 10.2147/OTT.S177325

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© 2018 Lu et al. This work is published and licensed by Dove Medical Press Limited

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miR-671-3p directly targets CKAP4.

Notes: (A) Illustration of the binding sites of CKAP4 and miR-671-3p. (B) Effect of miR-671-3p on the luciferase activities of CKAP4 3′-UTR in HEK-293T and SHG44 cells by luciferase reporter assay. (C) Expression of CKAP4 in miR-671-3p overexpressing or depleting cells by Western blot. (D) Statistical diagram of the Western blot results. (E) Expressions of CKAP4 in various glioma cells by Western blot. (F) Statistical diagram of (E). Error bars represent mean ± SEM. *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: CKAP4, cytoskeleton-associated protein 4; SEM, standard error of the mean; NC, negative control.