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. 2018 Sep 25;11:6217–6226. doi: 10.2147/OTT.S177325

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© 2018 Lu et al. This work is published and licensed by Dove Medical Press Limited

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Overexpression of CKAP4 impairs miR-671-3p-induced promotive effects on glioma cell behavior.

Notes: (A) Effect of reintroduction of CKAP4 on miR-671-3p-induced proliferation in A172 and SHG44 cells by CCK-8 assay (P<0.001 for A172; P<0.001 for SHG44). (B) Effect of CKAP4 on miR-671-3p-induced cell migration by Boyden Transwell assay (P<0.001 for A172; P<0.001 for SHG44). (C) Effect of CKAP4 on miR-671-3p-induced A172 and SHG44 cell apoptosis by flow cytometry (P<0.001 for A172; P<0.001 for SHG44). B1–B4 represent dead cells, late apoptosis cells, early apoptosis cells and alive cells, respectively. Error bars represent mean ± SEM. *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: CCK-8, Cell Counting Kit-8; CKAP4, cytoskeleton-associated protein 4; FITC, fluorescein isothiocyanate; SEM, standard error of the mean; NC, negative control.

Overexpression of CKAP4 impairs miR-671-3p-induced promotive effects on glioma cell behavior.

Notes: (A) Effect of reintroduction of CKAP4 on miR-671-3p-induced proliferation in A172 and SHG44 cells by CCK-8 assay (P<0.001 for A172; P<0.001 for SHG44). (B) Effect of CKAP4 on miR-671-3p-induced cell migration by Boyden Transwell assay (P<0.001 for A172; P<0.001 for SHG44). (C) Effect of CKAP4 on miR-671-3p-induced A172 and SHG44 cell apoptosis by flow cytometry (P<0.001 for A172; P<0.001 for SHG44). B1–B4 represent dead cells, late apoptosis cells, early apoptosis cells and alive cells, respectively. Error bars represent mean ± SEM. *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: CCK-8, Cell Counting Kit-8; CKAP4, cytoskeleton-associated protein 4; FITC, fluorescein isothiocyanate; SEM, standard error of the mean; NC, negative control.