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Expression of Fgfr2 and Pdgfra in E3.0 and E3.5 preimplantation embryos. Note, while Fgfr2mCherry and PdgfraH2B-GFP are differentially expressed in ICM cells at E3.0, both receptors are expressed in the same cells in E3.5 expanded embryos (E3.5 + 48h). Green background fluorescence in E3.0 and E3.5 embryos is due to the presence of the Zona Pellucida that surrounds the embryos. *, blastocoel cavity. Scale bars, 50 μm.
