| T-liposomes (500 μl) | ||
|---|---|---|
| final concentration | volume (μl) | |
| Syntaxin-1 (36 μM) | 5 μM | 69.4 |
| βOG 1 (20 %) | ~1% | 3.3 |
| SNAP-25 (200 μM) | 25 μM | 62.5 |
| TCEP (1 mM) | 1 μM | 0.5 |
| Protease inhibitors (200×) | 1× | 2.5 |
| Incubate the above reagents at r.t. for 25 min | ||
| Lipids for T-liposomes (10 mM) | 4 mM | 200.0 |
| βOG 2 (20 %) | ~1% | 6.0 |
| PhycoE-Biotin (16.7 μM) | 4 μM | 119.8 |
| HEPES buffer with 1 % βOG | 36.0 | |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.