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. Author manuscript; available in PMC: 2018 Sep 29.
Published in final edited form as: Nat Protoc. 2017 Aug 31;12(9):2014–2028. doi: 10.1038/nprot.2017.068
T-liposomes (500 μl)
final concentration volume (μl)
Syntaxin-1 (36 μM) 5 μM 69.4
βOG 1 (20 %) ~1% 3.3
SNAP-25 (200 μM) 25 μM 62.5
TCEP (1 mM) 1 μM 0.5
Protease inhibitors (200×) 2.5
Incubate the above reagents at r.t. for 25 min
Lipids for T-liposomes (10 mM) 4 mM 200.0
βOG 2 (20 %) ~1% 6.0
PhycoE-Biotin (16.7 μM) 4 μM 119.8
HEPES buffer with 1 % βOG 36.0