| V-liposomes (500 μl) | ||
|---|---|---|
| final concentration | volume (μl) | |
| Lipids for V-liposomes (10 mM) | 4 mM | 200.0 |
| Synaptobrevin (32 μM) | 8 μM | 125.0 |
| βOG (20 %) | ~1% | 6.4 |
| Cy5-Streptavidin (32 μM) | 8 μM | 125.0 |
| HEPES buffer with 1 % βOG | 40.6 | |
| TCEP (1 mM) | 1 μM | 0.5 |
| Protease inhibitors (200×) | 1× | 2.5 |
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