V-liposomes (500 μl) | ||
---|---|---|
final concentration | volume (μl) | |
Lipids for V-liposomes (10 mM) | 4 mM | 200.0 |
Synaptobrevin (32 μM) | 8 μM | 125.0 |
βOG (20 %) | ~1% | 6.4 |
Cy5-Streptavidin (32 μM) | 8 μM | 125.0 |
HEPES buffer with 1 % βOG | 40.6 | |
TCEP (1 mM) | 1 μM | 0.5 |
Protease inhibitors (200×) | 1× | 2.5 |