| VSyt1-liposomes (500 μl) | ||
|---|---|---|
| final concentration | volume (μl) | |
| Synaptotagmin-1 (18 μM) | 2.5 μM | 69.4 |
| βOG 1 (20 %) | ~1% | 1.0 |
| KCl 1 (3000 mM) | 600 mM | 20.5 |
| Synaptobrevin (32 μM) | 5 μM | 78.1 |
| TCEP (1 mM) | 1 μM | 0.5 |
| Protease inhibitors (200×) | 1× | 2.5 |
| Incubate the above reagents on ice for 15 min | ||
| βOG 2 (20 %) | ~1% | 9.5 |
| KCl 2 (3000 mM) | 600 mM | 65.6 |
| Lipids for VSyt1-liposomes (10 mM) | 2.5 mM | 125.0 |
| Cy5-Streptavidin (32 μM) | 8 μM | 125.0 |
| HEPES buffer with 1 % βOG | 2.9 | |
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