Skip to main content
. Author manuscript; available in PMC: 2019 Mar 2.
Published in final edited form as: Chembiochem. 2018 Jan 31;19(5):470–477. doi: 10.1002/cbic.201700542

Figure 3.

Figure 3.

Bioluminescent photon production from mutant luciferase enzymes and luciferin analogs. A) Analogs 3-4 (1000 μM) or D-luciferin (D-luc) were incubated with mutant luciferases (24 or 166) or native Fluc and ATP. Light emission was measured and quantified. Error bars represent the standard error of the mean for n ≥ 3 experiments. B) HEK 293 cells expressing mutant luciferases (24 or 166) or native Fluc were incubated with analogs 3-4 (1000 μM) or D-luciferin (D-luc). Error bars represent the standard error of the mean for n ≥ 3 experiments. For A) and B), p-values (from one-tailed unpaired t tests) are annotated by asterisks (*p < 0.05, **** p < 0.0001).