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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Acta Biomater. 2018 Aug 29;79:96–112. doi: 10.1016/j.actbio.2018.08.017

Figure 4: Spatial differences in the development of angular displacement of collagen fibril macrostructure in the anterior stroma during development.

Figure 4:

3D Directionality analysis of the stromal collagen organization with development. Collagen alignment in the developing corneal stroma was quantified by the Directionality plugin in Image J. The percentage of signal/collagen aligned at each angle from −90° to +90° within the image was evaluated and plotted against corneal stromal depth. At E9, the peaks show a preferred alignment of collagen at −90°, 0° and +90°, indicating an orthogonal/non-rotational collagen organization with respect to the choroid fissure (0°). At E10 onwards, the peaks display a shift from 0° illustrating the rotation of collagen at the anterior stroma. In the posterior stroma no shift in the peaks of the preferred collagen alignment is observed, illustrating that there is no rotation in the posterior stroma. Representative data from three samples for each embryonic stage.