Ex ovo chick corneal development at E15. (A) Chick eye showing retention of tube implanted at E3 (arrow). (B) Control, opposite eye of chick with tube implanted at E3. (C) Gross size of intubated eye. (D) Gross size of control, opposite eye. (E) Manually segmented stacks of 2D FFTs showing collagen rotation in the intubated eye and (F) in the control, opposite eye. Note that the intubated eye is markedly smaller, but has the same degree of collagen rotation indicating that IOP does not affect collagen organization. Eye intubation experiments were performed three times, one dozen eggs per experiment.