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. Author manuscript; available in PMC: 2018 Dec 29.
Published in final edited form as: Lab Invest. 2018 Jun 29;98(10):1347–1359. doi: 10.1038/s41374-018-0090-z

Figure 3.

Figure 3

TNF-α and VEGF decrease PrPc mRNA in endothelial cells. (A) BMVEC were treated with TNF-α (10ng/ml) for 12h, 18h, or 24h and mRNA levels of PrPc were evaluated using qRT-PCR. TNF-α decreased PrPc mRNA at 12h and 18h by 0.2 fold (20%) and 0.4 fold (40%), respectively, and PrPc mRNA returned to baseline at 24h, n=5. (B) BMVEC were treated with VEGF (100ng/ml) for 12h, 18h, or 24h and mRNA levels of PrPc were evaluated using qRT-PCR. VEGF decreased PrPc mRNA by 0.4 fold (40%), 0.5 fold (50%), and 0.6 fold (60%) at 12h, 18h, and 24h respectively, n=3. Significance was determined using a two-tailed paired Student’s t test. *p < 0.05, **p < 0.01, ***p< 0.0005.