Figure 4: Resolution enhancements obtained from bad particle removal.
A. Beta galactosidase map refined using all 19,829 particles, B. using only the “good” particle subset (15,211), and C. using only the “bad” particle subset (4,618). D. Gold standard FSC curves for each of the three maps in A-C. E. FSC between the maps in A-C and a 2.2Å PDB model (5A1A) of beta galactosidase. Note that the “good” particle subset has a better resolution both by “gold standard” FSC and by comparison to the ground-truth, indicating that the removed particles had been actively degrading the map quality.