Table 1.
Time (min) | Flow rate (mL/min) | Mobile Phase A (%) | Mobile Phase B (%) |
---|---|---|---|
0 | 0.5 | 80 | 20 |
0.8 | 0.5 | 30 | 70 |
2.8 | 0.5 | 5 | 95 |
3.8 | 0.5 | 5 | 95 |
4.0 | 0.5 | 60 | 40 |
5.5 | 0.5 | 60 | 40 |
Time (min) | Flow rate (mL/min) | Mobile Phase A (%) | Mobile Phase B (%) |
---|---|---|---|
0 | 0.5 | 80 | 20 |
0.8 | 0.5 | 30 | 70 |
2.8 | 0.5 | 5 | 95 |
3.8 | 0.5 | 5 | 95 |
4.0 | 0.5 | 60 | 40 |
5.5 | 0.5 | 60 | 40 |