Table 6:
Stability of CLBQ14 in Sprague Dawley Rat Plasma Samples for LC-MS/MS Analysis [n = 3; mean (± SD)]
| Biological Matrix | Time | Mean Recovery ± SD | |
|---|---|---|---|
| Short-term/Long-term Stability | Plasma | 2h | 91.6 ± 4.06 |
| 4h | 95.1± 3.24 | ||
| 6h | 93.7 ± 1.17 | ||
| 14 days | 91.3 ± 5.45 | ||
| Urine | 2h | 94.4 ± 1.83 | |
| 4h | 93.2 ± 4.10 | ||
| 6h | 95.6 ± 1.44 | ||
| 14 days | 101.6 ± 4.29 |
| Time (hr) | Mean Recovery ± SD | |||
|---|---|---|---|---|
| No IS | With IS | |||
| Processed sample or Auto-sampler stability | Plasma | 2 | 97.8 ± 1.11 | 95.9 ± 1.87 |
| 4 | 97.1 ± 5.42 | 94.7 ± 4.20 | ||
| 6 | 95.5 ± 6.69 | 97.3 ± 5.55 | ||
| Urine | 2 | 92.3 ± 1.32 | 102.1 ± 1.35 | |
| 4 | 92.8 ± 2.31 | 99.5 ± 1.89 | ||
| 6 | 94.2 ± 1.59 | 103.2 ± 2.33 | ||
| Nominal Concentration (ng/mL) | Mean Recovery ± SD | ||
|---|---|---|---|
| Freeze-thaw Cycle Stability | Plasma | 2.5 | 104.0 ± 4.72 |
| 400 | 95.9 ± 1.89 | ||
| 800 | 96.8 ± 1.25 | ||
| Urine | 2.5 | 95.5 ± 2.51 | |
| 400 | 87.4 ± 2.57 | ||
| 800 | 87.6 ± 2.26 |