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. Author manuscript; available in PMC: 2019 Mar 12.
Published in final edited form as: Nature. 2018 Sep 12;561(7724):547–550. doi: 10.1038/s41586-018-0515-2

Extended Data Figure 10. Reinforcement or without induction of tactile allodynia in animals with or without SNI by optogenetic stimulation of somatosensory CSNs.

Extended Data Figure 10

a, Schematic drawing of experimental paradigm. b-c, Measurement of punctate (von Frey), dynamic (brush) mechanical allodynia upon opto-stimulation in control (n=6) and ChR2-YFP+ CSNs (n=6) mice after SNI. n.s. and *, no statistical significance and P < 0.05, For b and c, P=0.18, 0.08 and 0.41, 0.02 for PLAP and Cre, without or with laser, respectively, two-sided student’s t-test. d, Representative images and quantification of pERK (red) and NK1R (green) immunostaining in the superficial dorsal horn (laminae I-II) of the spinal cord (L3-L4) in control (n=3) or hindlimb ChR2-YFP+ CSNs (n=3) animals receiving SNI and brush, coupled with opto-stimulation. Scale bar: 100 μm. *, P < 0.05, P=0.02 and 0.01 for pERK and pERK/NK1R ratio, respectively, two-sided student’s t-test. 6 sections crossing the lumbar spinal cord (L3-L4) were quantified for individual animals. Data are presented as mean ± SEM.