Figure 7.

Effects of vitamin D analogs: (A) 1,25(OH)₂D₃, (B) calcipotriol or (C) 21(OH)pD₃ at 0 (C—control), 0.01, 0.1 or 1 μM concentrations on VDR translocation to the nucleus (green fluorescence shown in a gray scale). Melanoma A375 cells were transduced using pLenti-CMV-VDR-GFP-pgkpuro construct: see Materials and Methods. Vitamin D analogs except 21(OH)pD induce VDR translocation to the nucleus after 1 h of incubation in a concentration-dependent manner. Each panel shows four random micrographs for each concentration taken by fluorescent microscope under 100× magnification.