Figure 2.

Quantitative RT-PCR of TRP channels in human endometrial stromal cells (hESC) derived from controls and endometriosis patients. Primary hESC were cultured from freshly isolated endometrium obtained during the luteal phase of controls (n = 3) and grade II endometriosis patients [37] (n = 4). mRNA levels were quantified to the geometric mean of housekeeping genes HPRT1 and PGK-1. TRPV6, TRPM2, TRPM3, and TRPM6 were around (30 < Cq < 35) or below the detection limit (Cq > 35). nd: not detectable. Data are presented as mean + SEM. Statistically significant changes in mRNA expression were assessed using the Two-way ANOVA statistical test with Bonferroni correction.