Table 2.
Primary and secondary antibodies.
| Primary Antibodies | ||||
| Antigen | Manufacturer | Catalog No. | Dilution | |
| Beta-actin | Sigma-Aldrich | A5441 | 1:20,000 | |
| Akt (pan) | Cell Signaling Technology | 4691 | 1:1000 | |
| Phospho-Akt (Ser473) | Cell Signaling Technology | 4060 | 1:2000 | |
| ERK1/2 | Cell Signaling Technology | 4695 | 1:1000 | |
| Phospho-ERK1/2 (Thr202/Tyr204) | Cell Signaling Technology | 4370 | 1:2000 | |
| MEK1/2 | Cell Signaling Technology | 9122 | 1:1000 | |
| Phospho-MEK1/2 (Ser217/221) | Cell Signaling Technology | 9121 | 1:1000 | |
| EGFR | Cell Signaling Technology | 2646 | 1:1000 | |
| Phospho-EGFR (Tyr1068) | Cell Signaling Technology | 2236 | 1:1000 | |
| PDGFR-beta | Cell Signaling Technology | 3169 | 1:1000 | |
| Phospho-PDGFR-beta (Tyr751) | Cell Signaling Technology | 4549 | 1:1000 | |
| Secondary antibodies | ||||
| Specificity | Conjugate | Manufacturer | Catalog No. | Dilution |
| Anti-Mouse IgG | horseradish peroxidase | Cell Signaling Technology | 7076 | 1:2000–1:20,000 |
| Anti-Rabbit IgG | horseradish peroxidase | Cell Signaling Technology | 7074 | 1:2000 |