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. 2018 Sep 11;16(9):328. doi: 10.3390/md16090328

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of PYCP supplementation on the activation and translocation of FoxO1 and FoxO3a in gastrocnemius muscle. (A) p-FoxO1, FoxO1, p-FoxO3a, and FoxO3a protein levels were examined by Western blot analysis. GAPDH was used as an internal standard; (B) FoxO1 and FoxO3a levels were measured in cellular cytosolic and nuclear fractions by Western blot analysis. β-actin, and lamin B were used as internal standards. Data represent mean ± standard deviation of three independent experiments (n = 5/group). * p < 0.05 vs. the corresponding control group; ^# p < 0.05 vs. the corresponding DEX-only treatment group.

Effects of PYCP supplementation on the activation and translocation of FoxO1 and FoxO3a in gastrocnemius muscle. (A) p-FoxO1, FoxO1, p-FoxO3a, and FoxO3a protein levels were examined by Western blot analysis. GAPDH was used as an internal standard; (B) FoxO1 and FoxO3a levels were measured in cellular cytosolic and nuclear fractions by Western blot analysis. β-actin, and lamin B were used as internal standards. Data represent mean ± standard deviation of three independent experiments (n = 5/group). * p < 0.05 vs. the corresponding control group; ^# p < 0.05 vs. the corresponding DEX-only treatment group.