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. 2018 Sep 11;16(9):328. doi: 10.3390/md16090328

Figure 7.

Figure 7

Effects of PYCP supplementation on the ubiquitin-proteasome system in gastrocnemius muscle. (A,B) Atrogin-1/MAFbx and MuRF1 mRNA and protein levels were assessed by real-time PCR and Western blot analysis. GAPDH was used as an internal standard; (C) 20S proteasomal activity was assessed by the detection of AMC in gastrocnemius muscle post cleavage from the peptide LLVY-AMC. Data represent mean ± standard deviation of three independent experiments (n = 5/group). * p < 0.05, ** p < 0.01 vs. the corresponding control group; # p < 0.05, ## p < 0.01 vs. the corresponding DEX-only treatment group.