Table 2.
Primary antibodies (monoclonal or polyclonal) used in the western blot analysis.
| Antibody | Manufacturer and Catalog No. | Species of Origin | Dilution Rate |
|---|---|---|---|
| 4E-BP1 | Santa Cruz Biotechnology: sc-9977 | Mouse | 1:1000 |
| Akt | Santa Cruz Biotechnology: sc-8312 | Rabbit | 1:1000 |
| ATG5 | Santa Cruz Biotechnology: sc-33210 | Rabbit | 1:1000 |
| ATG7 | Santa Cruz Biotechnology: sc-376212 | Mouse | 1:1000 |
| ATG12 | Santa Cruz Biotechnology: sc-271688 | Mouse | 1:1000 |
| Atrogin-1/MAFbx | Santa Cruz Biotechnology: sc-27645 | Goat | 1:2000 |
| β-actin | Santa Cruz Biotechnology: sc-47778 | Mouse | 1:1000 |
| BECN1 | Santa Cruz Biotechnology: sc-11427 | Rabbit | 1:1000 |
| Cathepsin-L | Santa Cruz Biotechnology: sc-6498 | Goat | 1:1000 |
| eIF4E | Santa Cruz Biotechnology: sc-514875 | Mouse | 1:1000 |
| FoxO1 | Santa Cruz Biotechnology: sc-374427 | Mouse | 1:500 |
| FoxO3a | Santa Cruz Biotechnology: sc-9813 | Goat | 1:1000 |
| GAPDH | Santa Cruz Biotechnology: sc-25778 | Rabbit | 1:1000 |
| IGF-IR | Santa Cruz Biotechnology: sc-713 | Rabbit | 1:1000 |
| IRS-1 | Santa Cruz Biotechnology: sc-560 | Rabbit | 1:1000 |
| Lamin B | Santa Cruz Biotechnology: sc-377000 | Mouse | 1:1000 |
| LC3-I/II | Cell Signaling: #4108S | Rabbit | 1:1000 |
| mTOR | Santa Cruz Biotechnology: sc-8319 | Rabbit | 1:1000 |
| MuRF1 | Santa Cruz Biotechnology: sc-27642 | Goat | 1:2000 |
| p62/SQSTM1 | Cell Signaling: #5114S | Rabbit | 1:1000 |
| p-4E-BP1 | Santa Cruz Biotechnology: sc-293124 | Mouse | 1:1000 |
| p70S6K | Santa Cruz Biotechnology: sc-8418 | Mouse | 1:1000 |
| p-Akt | Santa Cruz Biotechnology: sc-135650 | Mouse | 1:500 |
| p-FoxO1 | Cell Signaling: #9461S | Rabbit | 1:500 |
| p-FoxO3a | Cell Signaling: #9466S | Rabbit | 1:1000 |
| p-IGF-IR | Santa Cruz Biotechnology: sc-101703 | Rabbit | 1:1000 |
| p-IRS-1 | Santa Cruz Biotechnology: sc-17200 | Goat | 1:1000 |
| p-mTOR | Santa Cruz Biotechnology: sc-293132 | Mouse | 1:1000 |
| p-p70S6K | Santa Cruz Biotechnology: sc-8416 | Mouse | 1:1000 |
| p-S6 | Santa Cruz Biotechnology: sc-293144 | Mouse | 1:1000 |
| Raptor | Santa Cruz Biotechnology: sc-81537 | Mouse | 1:1000 |
| Rheb | Santa Cruz Biotechnology: sc-271509 | Mouse | 1:1000 |
| Rictor | Santa Cruz Biotechnology: sc-81538 | Mouse | 1:1000 |
| S6 | Santa Cruz Biotechnology: sc-74459 | Mouse | 1:1000 |