Figure 5.
ω3 fatty acids prevent FOX3a and BAD phosphorylation in vitro and in vivo. (a,b) HPDE and HPDE-KRAS cells were incubated with fixed doses of 40 µM of the ω3 fatty acids DHA and EPA, or the ω6 fatty acid LA. Expression of the AKT-target pFOXO3a was assessed by Western blotting. (c) pFOXO3a expression was next evaluated in the KRASG12D mice given control, ω3-, or ω6-enriched diets for 11 months. (d–f) HPDE and HPDE-KRAS cells incubated with DHA, EPA, or LA and similarly evaluated for expression of the AKT-target pBAD. Additionally, pBAD expression was measured in KRAS mice fed control, ω3, or ω6 diets (for 11 months).