Table 1.
The targets and primers designed for BnWRKY11 and BnWRKY70 and mutation rates in T0 plants.
| Target Gene (Number of Transformants) | Copies | Target | Amplification Primer | No. of Plants with Mutations | Mutation Frequency (%) |
|---|---|---|---|---|---|
| BnWRKY1122 | BnaA.WRKY11.a | WRKY11-Tgt1, WRKY11-Tgt3 |
11subF/11subR→11F/11R | 0, 7 | 0, 31.8% |
| BnaC.WRKY11.a | WRKY11-Tgt2, WRKY11-Tgt3 |
11F/11R | 12, 9 | 54.5%, 40.9% |
|
| BnWRKY708 | BnaC.WRKY70.b | WRKY70-Tgt1 | 70F3/70R3 | 0 | 0 |
| BnaA.WRKY70.b | WRKY70-Tgt1 | 70F3/70R3 | 3 | 37.5% | |
| BnaC.WRKY70.a | WRKY70-Tgt2 | 70F1/70R2 | 0 | 0 | |
| BnaA.WRKY70.a | WRKY70-Tgt3 | 70F1/70R1 | 3 | 37.5% |
Tgt, the target sequence used to generate sgRNA expression cassette. The amplify of BnaA.WRKY11.a was performed with the primer pair 11subF1/11subR1 first, then subcloned the products with primer pair 11F/11R.