Table 1.

Primers used for 16S rRNA gene library preparation. Primer names are indicated in the first column, and different regions of the primers are separated into columns for ease of viewing.

Primer Name	Illumina Adaptor Region	Phaser	Priming Region
PCR1_forward	GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT	(0–3 bp)	ACTCCTACGGGAGGCAGCAG
PCR1_reverse	ACACTCTTTCCCTACACGACGCTCTTCCGATCT	(0–3 bp)	GGACTACHVGGGTWTCTAAT
	Illumina Flowcell Region	Sample Index	Illumina Adaptor Region
PCR2_forward	CAAGCAGAAGACGGCATACGAGAT	(8 bp index)	GTGACTGGAGTTCAGACGTG
PCR2_reverse	AATGATACGGCGACCACCGAGATCT	(8 bp index)	ACACTCTTTCCCTACACGA