Figure 3.

Collagen type I expression in femurs. (A) Collagen expression of cortical bone was visualized with picrosirius staining, and photomicrographs were made by polarization microscope. Original magnification was 20×. Scale bar: 200 µm. Numbers below the photos show the differences of concentric lamellas thickness. (B) Immunohistochemistry of collagen type I in cortical bones. Original magnification was 20×. Scale bar: 200 µm. mRNA (C) and protein (D) expression of collagen type I. For RT-PCR and Western blot reactions, actin was used as controls. Optical signal density was measured and results were normalised to the controls. For panels (C,D) numbers below signals represent integrated signal densities determined by ImageJ software. Asterisks indicate significant (* p <0.05) alteration of expression compared to the respective controls. Representative data of 3 independent experiments.