Table 2.
Tables of antibodies used in the experiments.
| Antibody | Host Animal | Dilution | Distributor |
|---|---|---|---|
| Anti-PAC1 | rabbit, polyclonal, | 1:600 | Sigma-Aldrich, St. Louis, MO, USA |
| Anti-VPAC1 | rabbit, polyclonal, | 1:800 | Alomone Labs., Jerusalem, Israel |
| Anti-VPAC2 | rabbit, polyclonal, | 1:600 | Abcam, Camridge, UK |
| Anti-Coll. I. | mouse, monoclonal, | 1:1000 | Sigma-Aldrich, St. Louis, MO, USA |
| Anti-CREB | rabbit, polyclonal, | 1:800 | Millipore, Billerica, MA, USA |
| Anti-P-CREB | rabbit, polyclonal, | 1:800 | Millipore, Billerica, MA, USA |
| Anti-Osterix | rabbit, polyclonal, | 1:200 | SantaCruz Biotechnology Inc., Santa Cruz, CA, USA |
| Anti-Osteocalcin | rabbit, polyclonal, | 1:600 | Abcam, Camridge, UK |
| Anti-Osteopontin | rabbit, polyclonal, | 1:500 | Abcam, Camridge, UK |
| Anti-ALP | rabbit, polyclonal, | 1:500 | Abcam, Camridge, UK |
| Anti-SHH | rabbit, polyclonal, | 1:600 | Cell Signaling, Danvers, MA, USA |
| Anti-IHH | rabbit, polyclonal, | 1:600 | Millipore, Billerica, MA, USA |
| Anti-PKA | rabbit, polyclonal, | 1:800 | Cell Signaling, Danvers, MA, USA |
| Anti-Runx2 | rabbit, polyclonal, | 1:500 | Cell Signaling, Danvers, MA, USA |
| Anti-Gli1 | rabbit, polyclonal, | 1:600 | Cell Signaling, Danvers, MA, USA |
| Anti-BMP2 | mouse, monoclonal, | 1:500 | Abcam, Camridge, UK |
| Anti-BMP4 | rabbit, polyclonal, | 1:600 | Cell Signaling, Danvers, MA, USA |
| Anti-BMP6 | rabbit, polyclonal, | 1:200 | SantaCruz Biotechnology Inc., Santa Cruz, CA, USA |
| Anti-BMP7 | rabbit, polyclonal, | 1:600 | Abcam, Camridge, UK |
| Anti-BMPR1 | mouse, monoclonal, | 1:600 | Abcam, Camridge, UK |
| Anti-Smad1 | rabbit, polyclonal, | 1:600 | Cell Signaling, Danvers, MA, USA |
| Anti-PTCH1 | rabbit, polyclonal, | 1:800 | Cell Signaling, Danvers, MA, USA |
| Anti-Actin | mouse, monoclonal, | 1:10,000 | Sigma-Aldrich, St. Louis, MO, USA |