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. 2018 Aug 28;19(9):2550. doi: 10.3390/ijms19092550

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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CD163, MRC1, and COX-2 immunoreactivity was unchanged with SC transplantation though lesional and perilesional disparities were observed. Immunoreactivity for CD163 (red) was similar among SCI control (A,B) and EGFP-SC-transplanted (green) (G,H,M,N) animals, while MRC1 (red) did show a reduction in immunoreactivity from the SCI control (C,D) within the lesion–SC implant (I,J,O,P) but not overall. No change in the immunoreactivity for COX-2 (red) was seen between SCI control (E,F) and EGFP-SC transplant groups (K,L,Q,R). Total innate immune cells identified using Iba1 (blue). Triple staining of EGFP, Iba1 and CD163, MRC1 or COX-2 are shown in the merged images (pink; (N,P,R)). Iba1 Images were acquired at 40× objective magnification. Scale bar = 50 μm.