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BPF does not increase viability and cell proliferation of Saos-2 cells. Semi-confluent cultures of human osteoblast-like cells (Saos-2) incubated with BPF 0.001-0.01-0.1 mg/mL for 24 h. (A) Cell viability determined by MTT assay. (B) Cell proliferation determined by counting the number of cells in each well. Data are represented as mean ± SD. Abbreviations: BPF, Bergamot Polyphenol fraction; MTT assay, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
