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. 2018 Sep 14;10(9):1305. doi: 10.3390/nu10091305

Figure 2.

Figure 2

BPF increases phERK 1/2 protein levels and regulates RUNX2, RANKL and COL1A mRNA expression levels on Saos-2 cells. (A) Semi-confluent cultures of human osteoblast-like cells (Saos-2) incubated with BPF 0.001-0.01-0.1 mg/mL for 10 min. Cell proteins were analyzed by Western blotting with antibodies specific to phosphorylated ERK1/2 and β-actin. Semi-confluent cultures of human osteoblast-like cells (Saos-2) were incubated with BPF 0.001-0.01-0.1 mg/mL for 24 h. Then, mRNA expression levels of (B) RUNX2, (C) RANKL and (D) COL1A were measured by RT-Pcr. Data were analyzed using the 2−ΔΔCq method and normalized to β-actin. Data are represented as mean ± SD. Statistical analysis: Student’s t-test vs. 0 * p < 0.05; ** p < 0.01; *** p < 0.001; Linear regression # p < 0.05; ## p < 0.01. Abbreviations: BPF, Bergamot Polyphenol Fraction; RUNX2, Runt-related transcription factor 2; RANKL, Receptor Activator of Nuclear factor-κB Ligand; COL1A, Type 1A collagen.